Abstract
SEVERAL groups have demonstrated independently, the presence of two isoaccepting species of Phe-tRNA in rat hepa-tcmas and only one in normal rat liver. By use of reversed phase chromatography on RPC-2 columns1 two groups2–4 demonstrated an early eluting, minor peak of Phe-tRNA in Morris hepatomas 5123C and 5123D, which was not seen in normal rat liver tRNA, while one group5,6 reported the same extra peak in hepatoma 9618A but not in 3924A. We too have observed this minor peak of Phe-tRNA in Morris hepatomas 5123D, 7777 and 7800 using RPC-2 chromatography. Figure 1a shows a typical RPC-2 pattern for these three hepatomas all of which had a minor Phe-tRNA peak containing 14–15% of the total acid-precipitable counts. Recently Gonano et al. reported a similar peak of Phe-tRNA in foetal rat liver which cochromatographs on RPC-2 with the minor peak from hepatoma 5123C7.
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