Hepatocyte growth factor protects human mesangial cells against apoptosis induced by lead damage.

Abstract

Lead is a kind of nephrotoxic metal which frequently threats human health. Hepatocyte growth factor (HGF) is a multifunctional growth factor that protects cell apoptosis. In this study, human mesangial cells (HMCs) were treated with a single HGF dose of 20 and 40 μl/ml in order to investigate the effect of HGF on proliferation and apoptosis ability of HMCs induced by lead acetate. In HGF-treated group, HMCs were incubated with HGF (20, 40 μl/ml) half an hour prior to lead inducing. After lead-induced damage 48 h, the proliferation of HMCs was measured by MTT assay, and the apoptosis was assessed by flow cytometry. RT-PCR was used to detect the expression of P53, Bcl-2, Bax, and caspase-3 mRNA. The expression of Bax protein was measured by Western blot analysis. The results showed that HGF inhibits proliferation of HMCs induced lead acetate in a dose-dependent manner (P < 0.05). HGF significantly promoted the proliferation of HMCs, and flow cytometry revealed that HGF can inhibit apoptosis of HMCs. RT-PCR and Western blot showed that P53, Bax, and caspase-3 expression decreased, while Bcl-2 expression increased. HGF may afford a protection to HMCs against lead-induced damage.