Hepatocyte growth factor and matrix metalloprotease 2 levels in synovial fluid of the knee joint are correlated with clinical outcome of meniscal repair

Abstract

BackgroundThe reoperation rate after primary meniscal repair is about 20%. Thus far, it has remained unclear whether there are distinct individual preconditions that may be associated with a better or worse outcome of this procedure. We therefore analysed typical biochemical mediators in the synovial fluid (SF) of patients with meniscus tear before arthroscopic meniscal refixation and correlated their concentrations to the occurrence of re-rupture after meniscus repair. MethodsIn this study, 48 patients with meniscus ruptures were included. SF samples were taken intraoperatively prior to arthroscopy. Multiplex enzyme-linked immunosorbent assay (ELISA)-based methods were used to measure hepatocyte growth factor (HGF), interleukin-18 (IL-18), matrix metalloproteinases (MMP) MMP-1, MMP-2, MMP-9 and MMP-13 in the SF. At follow-up, the patients were classified into two groups: surgical success and surgical failure. ResultsTen out of 48 patients (20.8%) had to undergo revision surgery after meniscal repair (surgical failure). The median HGF in the surgical failure group was 2.4-times higher than in the surgical success group (P = .006), and the median MMP-2 was 1.8 times higher (P = .017). Concentration levels of the other tested proteins were not correlated with the success or failure of the meniscus surgery. There was weak evidence that both markers are indicators of an unsatisfactory healing process for meniscal refixation. ConclusionThese results suggest that HGF and MMP-2 could serve as molecular markers to estimate the chances of healing success of meniscus repair and possibly to individualise therapy in meniscal surgery.