Abstract
We investigated viability of hepatitis E virus (HEV) identified in contaminated pork liver sausages obtained from France. HEV replication was demonstrated in 1 of 4 samples by using a 3-dimensional cell culture system. The risk for human infection with HEV by consumption of these sausages should be considered to be high.
Highlights
We investigated viability of hepatitis E virus (HEV) identified in contaminated pork liver sausages obtained from France
We confirmed that 1 sample of pork liver sausage that had positive test results for HEV RNA by real-time reverse transcription PCR (RT-PCR) contained viable HEV
We cultured 4 sausage samples in 2 different institutes by performing 3D cell-culture propagation of HEV; HEV replication was detected in the same sample independently in both institutes, and the replicated virus was shown to be infectious
Summary
We investigated viability of hepatitis E virus (HEV) identified in contaminated pork liver sausages obtained from France. HEV RNA was detected in the 3D cell culture supernatants of all 4 sausage samples up to 8 d postinfection (dpi). Thereafter, HEV RNA was detected only in the cells inoculated with the sample A homogenate (Figure 1); it was assumed that the signals from the other 3 samples represented residual inoculum or an abortive infection.
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