Abstract
BackgroundHepatitis B virus (HBV) genotype appears to show varying geographic distribution. Molecular epidemiological study of HBV in particular areas in Indonesia is still limited. This study was aimed to identify the prevalence of HBV genotype/subgenotype and mutations in basal core promoter (BCP) region in voluntary blood donors in Makassar, one of the biggest cities in east part of Indonesia.A total of 214 hepatitis B surface antigen (HBsAg)-positive samples were enrolled in this study. HBV genotype/subgenotype was identified by genotype-specific PCR method or direct sequencing of pre-S region. Mutations in BCP were identified by direct sequencing of the corresponding region.ResultsHBV/B and HBV/C were detected in 61.21% and 25.23% of the samples, while mix of HBV/B and HBV/C was found in 12.62% of the samples. Based on pre-S region, among HBV/B and HBV/C, HBV/B3 (95.00%) and HBV/C1 (58.82%) were predominant. Interestingly, HBV/D was identified in two samples (22.165.07 and 22.252.07). Complete genome sequences of two HBV/D strains (22.165.07 and 22.252.07) demonstrated that both strains belong to HBV/D6, and the divergence between the two strains were 1.45%, while divergences of both 22.165.07 and 22.252.07 strains with reference strain (AM422939/France) were 2.67%. A1762T/G1764A mutation was observed in 1.96% and 5.36%, whereas T1753V mutation was found in 2.94% and 1.79% of HBV/B and HBV/C, respectively.ConclusionHBV/B and HBV/C are dominant in Makassar, similar to most areas in Indonesia. Mutations in BCP which might be associated with severity of liver disease are less common.
Highlights
Hepatitis B virus (HBV) genotype appears to show varying geographic distribution
HBV/B and HBV/C are dominant in Makassar, similar to most areas in Indonesia
Mutations in basal core promoter (BCP) which might be associated with severity of liver disease are less common
Summary
Hepatitis B virus (HBV) genotype appears to show varying geographic distribution. Molecular epidemiological study of HBV in particular areas in Indonesia is still limited. This study was aimed to identify the prevalence of HBV genotype/subgenotype and mutations in basal core promoter (BCP) region in voluntary blood donors in Makassar, one of the biggest cities in east part of Indonesia. HBV genotype/subgenotype was identified by genotype-specific PCR method or direct sequencing of pre-S region. Hepatitis B virus (HBV) infection is a major health problem leading to significant morbidity and mortality worldwide. Virology Journal 2009, 6:128 http://www.virologyj.com/content/6/1/128 moderate to high endemicity of hepatitis B virus (HBV) infection [2], due to the lack of proper health facilities or poor economical status and less public awareness. HBV genotypes appear to show varying geographic distribution. The HBV/G has been found in France, Germany and United States [14,15,16]
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