Abstract
1. 1. Rats were pretreated with 3-methylcholanthrene in order to increase the activity of hepatic UDPglucuronosyltransferase (g9-nitrophenol). UDPglucuronosyltransferase was solubilized by trypsin digestion and digitonin treatment. Seventy to ninety per cent of the activity was recovered in the soluble fraction after centrifugation at 105,000g for 60 minutes. 2. 2. The enzyme was further purified by Sepharose 4 B gel filtration, after which the increase in the specific activity of the pooled preparation was more than 100-fold. 3. 3. The gel filtrated preparation was rather stable, maintaining about 70% of its activity after 6 days storage at 0–4°C. 4. 4. UDPGlucuronic acid pyrophosphatase activity was found to be about one-hundredth of that in native rat liver microsomes. 5. 5. The phospholipid peak was located in the middle of fractions having UDPglucuronosyltransferase activity.
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