Hepatic phase II biotransformation in C57Bl/KsJ db/ db mice: comparison to that in swiss webster and 129 REJ mice

Abstract

1. 1. Cytochrome P-450 concentrations were similar in male and female carrier ( db/+) and diabetic ( db/ db) mice. Benzphetamine N-demethylase and styrene oxide hydrolase activities were 47 and 65% lower in b/+ than in db/ db mice. 2. 2. UDP-Glucuronosyltransferase activity toward 1-naphthol, estrone and diethylstilbestrol was not different between db/ db and db/+ but was 40% higher in db/ db mice toward testosterone. 3. 3. Glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene and ethacrynic acid was 47 and 59% lower in db/ db mice than in male db/+ mice. Female db/+ mice had similar activities to those found in diabetic animals. 4. 4. The differences in enzyme activity between hyperinsulinemic and normal animals suggest that insulin can influence both phase I and phase II biotransformations. 5. 5. Enzyme activities in db/+ and db/ db mice were compared to those in 129 REJ and Swiss Webster mice.