Hepatic mixed-function oxidases in California flatfishes are increased in contaminated environments and by oil and PCB ingestion

Abstract

Hepatic mixed-function oxidases (MFOs) were measured in the bothid flatfishes Citharicthys sordidus and C. stigmaeus from relatively uncontaminated and from polluted coastal populations of California, USA, at various times of the year during 1979–1980 and in individuals fed crude oil and polychlorinated biphenyl-augmented food in the laboratory. For C. sordidus, aryl hydrocarbon hydroxylase (AHH) specific activity was generally highest around the Los Angeles County sewage outfall on the Palos Verdes Shelf, intermediate near the 7-mile Hyperion sewage outfall in Santa Monica Bay and around a petroleum seep in the Santa Barbara Channel, and lowest in relatively unpolluted Monterey Bay. For C. stigmaeus, which had about ten times less specific activity than the foregoing species, specimens from the Santa Barbara petroleum seep had significantly greater AHH specific activity than those from Monterey Bay. Fishes from contaminated environments also showed increases of microsomal proteins with molecular weights of 56, 54, 57 and 46×103; moreover, the content of cytochrome P-450 was elevated in specimens of C. sordidus from such environments. Augmentation of food with seep oil or polychlorinated biphenyls (PCBs) induced significant increases in the specific activity of AHH and amounts of microsomal proteins in C. stigmaeus. Thus, these two species of flatfishes are good candidates for monitoring biologically meaningful levels of petroleum and polychlorinated biphenyls in contaminated environments. Moreover, the mixed function oxidase pattern in fish populations from the Santa Barbara petroleum seep is evidently a functional adaptation to chronic intake of petroleum hydrocarbons.