Abstract
1. 1. Rainbow trout developed elevated hepatic metallothionein concentrations after 4 weeks in a solution containing zinc, copper and cadmium in a fixed ratio of 400:20:1. 2. 2. Resistance to a combination of these metals increased in proportion to the concentration to which they were exposed for 4 weeks. 3. 3. The concentration of copper but not zinc or cadmium in the low molecular weight proteins separated by gel filtration was related to the concentrations of metallothionein present. 4. 4. The combined toxicity of the metals in the mixture was additive.
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