Abstract

Atomic absorption spectrophotometry is considered the method of choice for hepatic iron quantification. The objective of the present study was to perform full validation assays of hepatic iron quantification by atomic absorption spectrophotometry, using a fast sample preparation procedure, following the guidelines from the International Conference on Harmonization. The following parameters were evaluated: specificity, linearity/range, precision, accuracy, limit of detection and limit of quantification. A good linear correlation was found (0.9948) in the concentration range evaluated (20‐ 120 ppb). The relative standard deviations were below 15% for accuracy, and below 10% for both day‐to‐day reproducibility and within‐days precision, and the repeatability of injections was 0.65%. Limit of detection was 2 ppb, and limit of quantification was 6 ppb. Fresh bovine liver tissue was used to evaluate the procedure of collecting samples by liver biopsies. These findings indicate that hepatic iron quantification by atomic absorption spectrophotometry can be reliably performed at the established conditions, and suggest the method is suitable for further use in clinical practice. Hepatic iron quantification by AAS is validated by the experiments performed in the present study.

Highlights

  • The determination of the hepatic iron concentration (HIC) is important for the diagnosis and research of hemochromatosis and secondary iron overload states [1,2]

  • Hereditary hemochromatosis is one of the most prevalent inherited diseases in Caucasian populations, and secondary iron overload states may be associated with a variety of causes of chronic liver disease, like viral hepatitis, alcoholic liver disease, post-transfusional states and nonalcoholic fatty liver disease

  • Wortmann et al / Hepatic iron quantification by atomic absorption spectrophotometry liver iron overload is associated with hepatocelular damage, fibrogenesis and progression to cirrhosis

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Summary

Introduction

The determination of the hepatic iron concentration (HIC) is important for the diagnosis and research of hemochromatosis and secondary iron overload states [1,2]. A.C. Wortmann et al / Hepatic iron quantification by atomic absorption spectrophotometry liver iron overload is associated with hepatocelular damage, fibrogenesis and progression to cirrhosis. Atomic absorption spectrophotometry (AAS) is considered the method of choice, and allows iron quantification using very small-sized samples [8]. It can either be done with flame burners or using a graphite furnace [9]

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