Hepatic hemosiderosis in buffalo fish ( Ictiobus spp.)

Abstract

Hepatocellular hemosiderosis was observed in several species of fish associated with mixed chemical pollution in a Mississippi River Basin ecosystem. Three species of buffalo fish—smallmouth buffalo ( Ictiobus bubalus), bigmouth buffalo ( Ictiobus cyprinellus), and black buffalo ( Ictiobus niger)—were collected from a contaminated site, Devil's Swamp, and a control site, Tunica Swamp, both near Baton Rouge, Louisiana. Liver, kidney and spleen were examined histologically. Hepatocellular iron overload was observed in smallmouth buffalo and bigmouth buffalo from Devil's Swamp. Smallmouth buffalo from Tunica Swamp had no stainable iron in the hepatocytes. Bigmouth buffalo from Tunica Swamp had trace amounts of stainable iron in the hepatocytes. Black buffalo from both Devil's Swamp and Tunica Swamp had no stainable iron in the hepatocytes. Iron could accumulate in sediment where it enters the food chain through benthic organisms. Analyses of water and sediment from both study sites revealed similarly high concentrations of iron. The pollutants in the Devil's Swamp may have affected the iron absorption and metabolism in smallmouth buffalo and bigmouth buffalo. The variation in the presence of stainable iron in the liver of three species of buffalo fish may indicate significant interspecies variation in the iron absorption and metabolism under conditions of mixed pollution.