Abstract

Hepatic glycogen synthesis fluxes from direct and indirect pathways were quantified in seabass by postmortem 2H NMR analysis of plasma water (PW) and glycogen glucosyl 2H enrichments from 2H-enriched seawater. Eighteen fish (28.0±1.7cm and 218.0±43.0g) were divided into three groups of 6 and studied over 24days with transfer to 5% 2H-seawater after day 21. Over this period, one group was fed daily with fishmeal, a second group was fasted, and a third group was fasted for 21days followed by 3days refeeding. Glycogen turnover and sources were determined from the ratio of glucosyl position 5 enrichment to that of plasma water (H5/PW). Glycogen levels of fed fish were significantly higher than fasted (665.4±345.2μmol.g−1 liver versus 77.2±59.5μmol.g−1 liver, P<0.05) while refed fish had comparable levels to fed (584.6±140.4μmol.g−1 liver). Glycogen enrichment of fed fish was undetectable indicating negligible turnover over 3days. For fasted fish, H5/PW was ~50% indicating that half of the glycogen had turned over via indirect pathway flux. For refed fish, H5/PW was ~100% indicating that the indirect pathway accounted for all net glycogen synthesis. Direct pathway conversion of dietary carbohydrate to glycogen was not detected in any of the groups.

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