Abstract
ObjectiveIncreased hepatic de novo lipogenesis (DNL) is suggested to be an underlying cause in the development of nonalcoholic fatty liver disease and/or insulin resistance. It is suggested that omega-3 fatty...
Highlights
Nonalcoholic fatty liver disease (NAFLD), defined as excess intrahepatocellular triacylglycerol (IHTAG) accumulation due to nonalcoholic causes, is a complication in individuals who are obese and/or have type
Findings from animal and in vitro work show omega-3 fatty acids (FA) have a hepatocyte-specific effect by downregulating glucose or D31 palmitate added to the culture media and cells and media were collected for the measurement of the effect of eicosapentaenoic acid (EPA)+docosahexaenoic acid (DHA) on: (C) the relative contribution (%) of glucose-derived de novo lipogenesis (DNL) FAs to intracellular TAG (n=4) and (D) FA oxidation as measured by 2H2O media (n=6)
Some have reported fasting hepatic DNL to be correlated with IHTAG content and very low-d ensity lipoprotein (VLDL)-T AG secretion rate,[4] we found no associations between change in IHTAG content and change in hepatic DNL
Summary
Whole blood was collected into heparinized syringes (Sarstedt, Leicester, UK) and plasma was rapidly separated by centrifugation at 4 oC for the measurement of plasma metabolite and insulin concentrations as described.[25] Separation of the chylomicron fraction ((Svedberg flotation rate,Sf)>400) and the very low-d ensity lipoprotein (VLDL)-rich fraction (Sf20-400) were made by sequential flotation using density gradient ultracentrifugation and anti-ApoB100 immunoabsorption capture to obtain a fraction completely devoid of apoB48 and hereafter called VLDL, as described.[25]. To determine the specific FA composition and isotopic enrichment, total lipids were extracted from plasma and lipoproteins. FA methyl esters were prepared from plasma NEFA, and TAG fractions, along with erythrocyte total phospholipids as described.[22] The FA compositions (μmol/100 μmol total FA) in these fractions were determined by gas chromatography (GC) and in plasma NEFA and TAG fractions palmitate concentrations were calculated as described.[25]
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