Abstract
The objectives of this study were to explore whether heparin-binding proteins, separated by fast protein liquid chromatography from boar seminal plasma influence the release of prostaglandins F2α, (PGF2α), E2 (PGE2) and interleukin-6 (IL-6) by porcine endometrial and cervical cells and even bovine endometrial cells. In Experiment I, we showed that release of PGF2α by endometrial epithelial, endometrial stromal and cervical stromal cells to the medium was inhibited (p α (TNFα) stimulated release of IL-6 by endometrial and cervical stromal cells after 24 h incubation, but in the presence of heparin-binding proteins, this stimulation was attenuated. Release of PGF2α by cryopreserved (Experiment II) and primary (Experiment III) cervical stromal cells was significantly inhibited after 3 h incubation with 66 - 95.4 μg of heparin- binding proteins. A significant inhibition of PGE2 release by cryopreserved and primary cervical stromal cells was already achieved after incubation with 16.5 - 23.9 μg of heparin-binding proteins. The release of IL-6 by cryopreserved cells was stimulated after 3 h incubation with heparin- binding proteins in a dose dependent manner in contrast to the release of IL-6 by freshly isolated cervical stromal cells. We also found (Experiment IV) that porcine heparin-binding seminal plasma proteins inhibited release of PGF2α and stimulated release of IL-6 by bovine endometrial epithelial cells. In conclusion, a group of heparin-binding proteins separated by fast protein liquid chromatography from boar seminal plasma inhibit PGF2α, PGE2 and stimulate IL-6 release by porcine endometrial and cervical cells and even by bovine endometrial cells. Thus, these proteins have a similar effect as the entire seminal plasma.
Highlights
The potent biological activity of boar seminal plasma to inhibit the release of prostaglandins and to stimulate IL-6 release by porcine endometrial, cervical and bovine endometrial cells has been demonstrated previously [1]
We demonstrated that heparinbinding proteins (F2) separated on fast protein liquid chromatography (FPLC) from boar SP have the ability to inhibit the release of PGF2α by porcine endometrial and cervical cells
We demonstrated that heparin-binding proteins from boar SP stimulate IL-6 release by porcine cervical stromal cells after 3 h incubation
Summary
The potent biological activity of boar seminal plasma to inhibit the release of prostaglandins and to stimulate IL-6 release by porcine endometrial, cervical and bovine endometrial cells has been demonstrated previously [1]. It is well known that prostaglandins F2α and E2, which are of endometrial origin, regulate a number of important physiological functions during the estrous cycle and pregnancy in pigs [2,3]. These two prostaglandins are involved in acute and chronic inflammatory conditions [4,5]. Seminal plasma suppresses the migration of polymorphonuclear neutrophil granulocytes (PMNs) into the uterus, decreases PMNs infiltration of the endometrium and enhances the rate of disappearance of uterine inflammation in gilts following breeding [6,7]. Porcine SP does not activate neutrophil chemotaxis, but it might play a different role in the mo-
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