Heparin suppression of gastric acid secretion.

Abstract

During gastric secretory studies in dogs designed to test the effect of cross-transfusion of a proposed antral chalone, we noted inhibition of gastric secretion following intravenous administration of heparin(1). The following experiments were designed to study the effect of this inhibition on gastric secretion stimulated by food (antral phase) and by histamine. Materials and methods. Thirteen adult female mongrel dogs weighing approximately 20 kg were used in this study. Denervated pouches of the gastric fundus (Heidenhain) were prepared in the usual fashion, and a stainless steel cannula was used to drain the pouch to the outside. During the 30 or more days which elapsed between the operation and onset of testing, the dogs were trained in the Pavlov stand. The animals were fasted for 18 or more hours prior to testing. In all studies, gastric pouch secretion was measured every 30 minutes and the acidity determined by using Töpfer's reagent, titrating with N/10 NaOH. Specimens of less than 1 cc were discarded. All animals were in a basal secretory state prior to testing. 1. Antral phase, food stimulus. Twenty-one paired control and test studies were performed on 5 dogs. The antral phase of gastric secretion was stimulated by feeding a standard test meal of 400 g of Pard. In control studies, 1 cc of physiologic saline solution was given intravenously at time of feeding. In test studies, 100 mg (1 cc) of aqueous heparin was given intravenously at time of feeding. Control and test studies were otherwise identical and were performed on alternate days. The sequence of control-test experiments was varied. Pouch secretions were collected for 4 hours following feeding of standard meal. 2. Histamine stimulus. Fifty-one paired control-test studies were performed on 8 dogs. One mg histamine base was injected subcutaneously and gastric secretions were collected from the Heidenhain pouch for 3 hours. In control studies, 1 cc of sterile saline solution was given intravenously at time of histamine administration, and in test studies, 100 mg (1 cc) of aqueous heparin was given intravenously at time of histamine administration.