Heparin Sodium Was Prepared from Pig Intestinal Mucosa by Dialysis and Spray Drying

Shunshun Shu,Qian Mi,Caixia Yang,Jun Niu,Zhaoyang Wang,Chonghuan Bao

doi:10.4236/jbm.2018.610002

Abstract

A method to extract crude heparin sodium from pig intestinal mucosa by dialysis and spray drying was established. The pig intestinal mucosa was treated in the following steps: enzymolysis, resin exchange adsorption-washing, elution, pressure filtration, dialysis, spray drying. Activity of the product was measured using a heparin anti-IIa factor assay kit. The yield of crude heparin obtained by this method was 2.79% higher than that of oven drying method; the production of 1 kg crude heparin sodium saved 43.4 pigs small intestine. The activity was 98.48 ± 2.49 IU/mg (n = 5), 15.18 IU/mg higher than that obtained by oven drying method. The product is pale white powder, attractive color and easy to dissolve.

Highlights

Heparin sodium is the sodium salt of heparin; it is a kind of natural mucopolysaccharide sulfate ester with complex structure extracted from biological materials
The yield of crude heparin obtained by this method was 2.79% higher than that of oven drying method; the production of 1 kg crude heparin sodium saved 43.4 pigs small intestine
Effect of Dialysis and Spray Drying on the Appearance of Crude Heparin Sodium

Summary

Introduction

Heparin sodium is the sodium salt of heparin; it is a kind of natural mucopolysaccharide sulfate ester with complex structure extracted from biological materials. Its basic framework is a polysaccharide chain linked by a glycosylation bond between uronic acid and glucosamine. It generally forms a complex with proteins in animals [1]. The structure of heparin sodium is generally considered as Figure 1. Heparin is a sulphated polysaccharide with a high affinity for antithrombin. Antithrombin exhibits a fast acting and potent inhibitory activity for coagulant serine esterases: IXa, Xa and thrombin. Low Molecular Weight Heparin (LMWH), and heparin analogues inhibit more efficiently

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