Abstract
The study evaluated the ameliorative effect of vitamin C on chronic chlorpyrifos-induced hematological alterations in Wistar rats. Twenty adult male rats divided into 4 groups of 5 animals each were exposed to the following regimens: group I (S/oil) was administered soya oil (2 mL/kg b.w.), while group II (VC) was given vitamin C (100 mg/kg b.w.); group III was dosed with CPF (10.6 mg/kg b.w.); group IV was pretreated with vitamin C (100 mg/kg) and then exposed to CPF (10.6 mg/kg b.w.), 30 minutes later. The regimens were administered by oral gavage once daily for a period of 17 weeks. Blood samples collected at the end of the study revealed reduction in the levels of pack cell volume, hemoglobin, red blood cells, leukocytes (attributed to neutropenia, lymphopenia, and monocytopenia), and platelets in the CPF group, which were ameliorated in the vitamin C- pretreated group. The elevated values of malonaldehyde, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and neutrophil/lymphocyte ratio in the CPF group were restored in those pretreated with vitamin C. The study has shown that chronic CPF-induced adversity on hematological parameters of Wistar rats was mitigated by pretreatment with vitamin C.
Highlights
Organophosphate (OP) insecticides are used in the agricultural and domestic pest control [1], accounting for 50% of the global insecticidal use [2]
The aim of the present study is to evaluate the mitigating effect of vitamin C on hematological changes induced by chronic CPF exposure in Wistar rats
The erythrocyte malonaldehyde (MDA) concentration, as a marker of lipid peroxidation, was determined by the double heating method of Draper and Hadley [18], as we described previously [4, 6]
Summary
Organophosphate (OP) insecticides are used in the agricultural and domestic pest control [1], accounting for 50% of the global insecticidal use [2]. Their use is, accompanied by widespread toxicity in nontarget organisms, including man. Chlorpyrifos (CPF) is one of the most widely used OP insecticides until 2000 when the United States Environmental Protection Agency restricted some of its domestic uses due to its toxicity. CPF passes through the cells into the cytoplasm [9]. CPF induces damage to the cellular molecules [10]
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