Abstract
The percentage of preservation of erythropoietic and granulopoietic precursor cells in the murine bone marrow was studied using in vitro methylcellulose clonal cell culture assays and in vivo murine spleen colony assays. This study clearly demonstrates a Type of Spleen Colonies Induced by 6-hr Postmortem Murine Bone Marrow Cells a Mean (%) Type of colonies t Score P Value Unfrozen Frozen Erythrocytic 26.283 14.100 2.09 0.059 Granulocytic 23.741 32.917 1.45 0.173 Mixed 49.321 52.700 0.55 0.59 a N = 92. the presence of pluripotent hemopoietic precursor cells in cryopreserved 0-, 3-, 6-, 9-, and 12-hr postmortem murine bone marrow cells. Apparently, the erythropoietic precursor cells are more sensitive to freezing injury as compared to granulopoietic precursor cells.
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