Hemolytic and Proteolytic Activities of Aeromonas hydrophila and Aeromonas veronii Biovar sobria in Broth and Salmon Extract at Different Temperatures

Abstract

Expression of hemolytic and proteolytic activities throughout the growth cycle was investigated with two enterotoxic aeromonad strains assigned to the species Aeromonas hydrophila and Aeromonas veronii biovar sobria. Although growth kinetic data were dependent on strain, temperature, and substrate, maximum populations attained were higher than 9 log CFU/ml in aerated tryptone soya broth plus yeast extract (TSBYE) and salmon extract within the range 4 to 28°C. For both strains in TSBYE, variable amounts of hemolytic activity were first detected at any temperature when aeromonad counts were over 9 log CFU/ml. Afterwards, this activity increased up to similar levels (109 to 112 hemolytic units per ml) without a significant increase in populations. Salmon extract supported hemolysin synthesis at 28 but not 4°C. Proteolytic activity of the A. hydrophila strain was only expressed in salmon extract at 28°C, whereas A. veronii biovar sobria did at 28°C in both substrates and at 10°C in TSBYE.