Abstract
Rat erythrocytes prepared as a 0.1% suspension were incubated at 37.5 °C, and the course of hemolysis was followed by either measuring the hemoglobin released to the medium or by recording the optical density of the suspension. The response of the cells to p-hydroxymercuribenzoate (PMB), N-ethylmaleimide (NEM), sodium glycocholate, glutathione, and glucose—either singly or in combination with each other—was observed. The results suggested that PMB hemolysis at low concentrations is the result of its sulfhydryl activity only because this reagent is capable of first releasing SH groups, and that the nonhemolytic effect of NEM is due to its inability to release such reactive SH groups.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
