Abstract
The hemolysis of horse, human and pig erythrocytes mediated by myeloperoxidase, lactoperoxidase and saliva peroxidase was studied using turbidimetry and microscopic analysis. The addition of myeloperoxidase, a hydrogen peroxide-generating system (glucose+glucose oxidase) and chloride or bromide caused the opaque suspensions of these mammalian erythrocytes to change into transparent (orange to yellowish green) solutions. Under the microscope, cells became dim circles and faded from the field of vision during the decrease in turbidity. Myeloperoxidase could be replaced by lactoperoxidase or saliva peroxidase in the system containing bromide, but not in that containing chloride. Hemolysis by these peroxidase systems was observed in the range of moderately acidic pH, with the optimum pH values being between 5.0 and 5.5. The present study also showed that turbidimetry and microscopic analysis are useful in assessing hemolysis by the peroxidase-hydrogen peroxide-halide systems.
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