Abstract
Objectives The aim of the study was to investigate hemoglobin (Hb) species in a 61 year-old male with diabetes mellitus type II and a low value of Hb A 1c. Design and methods Hb species were analyzed by electrophoresis and chromatography methods. Functional properties were determined by oxygen equilibrium studies. β-globin gene was amplified by PCR and sequenced. Results and conclusions A novel clinically silent Hb (Hb Seville), that results in falsely low Hb A 1c measurement, was detected. This Hb variant presented a single base mutation at codon 81 (C → T) of the β-globin gene. This case points out the necessity of careful inspection of the chromatograms and the use of additional methods to Hb A 1c measurement when the presence of aberrant peaks is detected.
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