Hemoglobin measurement in venous blood compared with pooled and single-drop capillary blood: a method-comparison study in a controlled and survey setting in Uganda among children and women

Abstract

BackgroundStandard practice for estimating anemia in population-based surveys is to use a point-of-care device to measure hemoglobin (Hb) in a single drop of capillary blood. Emerging evidence points to larger than expected differences in Hb concentration depending on the blood source. ObjectiveWe evaluated use of different blood sources to measure Hb with a HemoCue 201+ analyzer compared with the reference method of venous blood tested with a Sysmex XN-450 hematology analyzer. MethodsHb concentration in venous, pooled capillary, and single-drop capillary blood were collected in controlled (laboratory) and survey (Demographic Health Survey-8 pilot) settings in Uganda among children 6–59 mo and nonpregnant women 15–49 y. Venous and capillary blood collected from the same individual was tested using a HemoCue 201+ analyzer and the venous blood was also measured with a Sysmex XN-450 hematology analyzer. Agreement between measures was estimated using Lin’s concordance correlation coefficient, Bland–Altman plots, and Deming regression. Means and prevalences were compared using paired t-tests and McNemar’s tests, respectively. ResultsThe limits of agreement between Hb measured using a HemoCue 201+ analyzer and the reference method were lowest for venous (1.1–1.96 g/dL), followed by pooled capillary (1.45–2.27 g/dL), and single-drop capillary blood (2.23–3.41 g/dL). Mean differences were <0.5 g/dL across comparators. There were statistically significant differences in Hb concentration from both types of capillary blood. Anemia prevalence was lower in pooled capillary blood compared with the reference method. ConclusionsThe variability of Hb measured by capillary blood using the HemoCue 201+ analyzer is higher than venous blood but the extent to which this impacts the validity of Hb and anemia estimates requires further exploration. Future research is also needed to evaluate the implications of using venous compared with capillary blood in population-based surveys.This trial was registered at clinicaltrials.gov (NCT05059457).