Hemoglobin Level and Total Leukocyte Differential in Laying Hens Fed with Betel Leaf Juice (Piper betle Linn)

Abstract

This research aims to examine the hemoglobin level and the total leukocyte differential in the blood of laying hens fed with different levels of betel leaf juice (Piper betle Linn). There were 32 laying hens in layer phase. The cage used is an individual cage equipped with a place for feeding and drinking water. The treatments in this research consisted of: (P0) drinking water + 0 ml of betel leaf juice, (P1) drinking water + 5 ml of betel leaf juice, (P2) drinking water + 10 ml of betel leaf juice, and (P3) drinking water + 15 ml of betel leaf juice. The variables observed were hemoglobin level, leukocyte differential (lymphocytes, heterophils, monocytes, eosinophils and basophils) and the heterophils / lymphocyte ratio (H/L). The data obtained were analyzed using analysis of variance and then continued with Duncan's multiple range test. The results showed that the addition of betel leaf juice had no significant effect (p> 0.05) on the levels of hemoglobin, lymphocytes, heterophils, monocytes, eosinophils, basophils and the H/L ratio of laying hens’ blood. Based on the results of the research, it can be concluded that the addition of betel leaf juice at different levels does not affect the levels of hemoglobin, lymphocytes, heterophils, monocytes, eosinophils, basophils and the H/L ratio of laying hens’ blood, but these conditions are still in normal physiological conditions.