Hemoglobin Extracted From Perinereis aibuhitensis Acts as a Cell Culture Medium Supplement to Reduce Apoptosis

Qianyu Huo,Yunde Liu,Bin Cao,Jing Liu,You Cheng,Ming Lei

doi:10.3389/fmars.2021.659934

Abstract

The environmental oxygen concentration is a crucial factor affecting cell proliferation. Owing to the reversible binding property of hemoglobin to oxygen, it can be utilized to regulate the oxygen concentrationin vitro, and its ability to reduce apoptosis can be evaluated. In this study, a process comprising isolation, purification, and extraction was used to obtain hemoglobin fromPerinereis aibuhitensis, a polychaete invertebrate. Extracts were separated and characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Extract component identity was confirmed by matrix-assisted laser desorption time-of-flight mass spectrometry analysis, with the molecular weight determined as 412,216.6875 Da. The oxygen carrying capacity ofP. aibuhitensishemoglobin was comparable with that of human hemoglobin.P. aibuhitensishemoglobin remarkably downregulated the apoptosis rate. Reactive oxygen species (ROS) assays confirmed the reduction in ROS production, enabling a better elucidation of the mechanism underlying the decrease in apoptosis. These results suggested thatP. aibuhitensishemoglobin is a natural oxygen carrier, that, owing to its low-cost and accessibility, can be considered a candidate for culture medium supplement to reduce the apoptosis rate.

Highlights

Perinereis aibuhitensis (PA) is a polychaete invertebrate that inhabits the estuaries, mangroves, and mud flats common in tropical and subtropical Asia
0.625 μg/mL was selected as the appropriate concentration of PA Hb for the subsequent experiment performed to analyze cell apoptosis and detect Reactive oxygen species (ROS)
This study provides evidence to support that PA hemoglobin helps to suitably redistribute oxygen in cell culture systems

Summary

Introduction

Perinereis aibuhitensis (PA) is a polychaete invertebrate that inhabits the estuaries, mangroves, and mud flats common in tropical and subtropical Asia. It is widely distributed along the coasts and estuaries of the Northwest Pacific. PA has been increasingly used in marine ecotoxicological studies (Zhang et al, 2017; Jiang et al, 2019), a resultant of its important role in the fate of chemicals in the sediments owing to its biological distribution, roles in particle mixing and irrigation, and influence on metal forms (Cong et al, 2011; Tong et al, 2019). Biological agents extracted from PA have been used for antithrombotic food development (Li et al, 2017).

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