Abstract
Objectives1)To investigate the presence of hemoglobin Constant Spring (HbCS) in a patient with severe microcytic anemia who had previously been diagnosed with alpha thalassemia minor.2)To assess the stability of HbCS post blood collection by high performance liquid chromatography (HPLC). Design and methodsHemoglobin fractionation was performed by HPLC immediately after specimen collection using the β-thalassemia Short Program on the BioRad Variant II. To assess HbCS stability, the patient's specimen was re-analyzed over a 17day period. ResultsHPLC analysis showed a low abundance peak with chromatographic properties consistent with HbCS. Presence of this hemoglobin variant was confirmed by electrophoresis and gene sequencing. HbCS remained detectable by HPLC for 17days after specimen collection, with minimal degradation. ConclusionsOur results suggest that HbCS is stable many days after blood collection. Consequently, it is not necessary to analyze specimens immediately after collection when assessing the potential presence of this hemoglobin variant.
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