Abstract
1.An acrylamide disc electrophoretic method is described for separation of hemoglobins with direct quantitation by densitometry; the method is rapid, accurate, and convenient.2.By this method, measurement of hemoglobin A2 in 70 normal subjects gave a mean of 2.96%, range 2.1–3.9%. Twenty-one subjects with β-thalassemia trait gave a mean of 6.32%, range 4.4–8.4%.3.Hemoglobin F can be separated and quantitated by densitometry when present as more than 10% of the total. By this method, values for hemoglobin F are higher than those obtained by alkaline denaturation.4.The method also allows convenient determination of haptoglobin types and semi-quantitation of serum haptoglobin level. An acrylamide disc electrophoretic method is described for separation of hemoglobins with direct quantitation by densitometry; the method is rapid, accurate, and convenient. By this method, measurement of hemoglobin A2 in 70 normal subjects gave a mean of 2.96%, range 2.1–3.9%. Twenty-one subjects with β-thalassemia trait gave a mean of 6.32%, range 4.4–8.4%. Hemoglobin F can be separated and quantitated by densitometry when present as more than 10% of the total. By this method, values for hemoglobin F are higher than those obtained by alkaline denaturation. The method also allows convenient determination of haptoglobin types and semi-quantitation of serum haptoglobin level.
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