Abstract
A multi-functional imaging system capable of determining relative changes in blood flow, hemoglobin concentration, and morphological features of the blood vasculature is demonstrated. The system combines two non-invasive imaging techniques, a dual-wavelength laser speckle contrast imaging (2-LSI) and an optical microangiography (OMAG) system. 2-LSI is used to monitor the changes in the dynamic blood flow and the changes in the concentration of oxygenated (HbO), deoxygenated (Hb) and total hemoglobin (HbT). The OMAG system is used to acquire high resolution images of the functional blood vessel network. The vessel area density (VAD) is used to quantify the blood vessel network morphology, specifically the capillary recruitment. The proposed multi-functional system is employed to assess the blood perfusion status from a mouse pinna before and immediately after a burn injury. To our knowledge, this is the first non-invasive, non-contact and multifunctional imaging modality that can simultaneously measure variations of several blood perfusion parameters.
Highlights
The blood vasculature regulates the blood flow and tissue perfusion, and mediates the delivery of nutrients and oxygen to the living tissue [1]
We propose a newly developed multi-functional imaging system, which can simultaneously extract the information as to the changes in blood flow, concentration of oxy and deoxyhemoglobin and morphological change of the vasculatures
We have demonstrated that a multi-functional imaging system that combines 2. Dual-wavelength laser speckle imaging (2-LSI) with optical microangiography (OMAG) can be used to asses several important physiological parameters of the tissue blood perfusion status
Summary
The blood vasculature regulates the blood flow and tissue perfusion, and mediates the delivery of nutrients and oxygen to the living tissue [1]. A laser Doppler flowmetry system has been integrated with near-infrared oxymetry as a dual-wavelength system for simultaneous imaging of blood flow and hemoglobin oxygenation changes induced by cortical transient ischemia in rat brain [28]. We propose a newly developed multi-functional imaging system, which can simultaneously extract the information as to the changes in blood flow, concentration of oxy and deoxyhemoglobin and morphological change of the vasculatures. This system integrates a 2-LSI and OMAG into one single imaging system. The newly developed multi-functional system would be beneficial for studying and diagnosing the microvasculature related diseases
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