Hemocompatibility assessment and drug release kinetics investigation from materials based on electrospun nanofibers

Abstract

Objective(s): Polymeric nanofiber has a huge potential for a various biomedical applications such as wound healing and orthopedic implant. Since most of the future applications of therapeutic nanofiber are interacting with human blood components, it is important to investigate hemocompatibility. Methods: In this study, nanofibers with antibacterial properties were synthesizedby electrospun of polymeric composite of chitosan (CS), poly (ethylene oxide) (PEO) and vancomycin (vanco). The results obtained from scanning electron microscopy (SEM), FTIR spectroscopy, antibacterial and hemolysis tests of nanofiber were evaluated. The kinetic and drug release mechanism of drug loaded electrospun samples were also investigated by UV-Vis spectrophotometry and the appropriate model was proposed for prediction of drug release.Results: The surface morphology of a composite nanofiber indicated that the nanofiber is flat and smooth. The results of antibacterial tests showed that prepared nanofiber has antibacterial properties against of Staphylococcus aureus and Escherichia coli. Hemolysis test indicate that this nanofiber has non hemolytic impact on red blood cells (RBCs). The model of antibiotic release from the nanofiber was examined and it was found that the release mechanism can be described as Fickian diffusion model. According to this model, the kinetic degree of the drug release is around 0.38.Conclusions: Infections and pathophysiological factors cause delayed healing of wound healing. Therefore, using antibacterial nanofibers for elimination of antibacterial infection from wounds, accelerate wound healing.