Abstract
Publisher Summary The ES/EB system has been instrumental in identifying a progenitor with hemangioblast properties. When early EB-derived cells are cultured in the presence of vascular endothelial growth factor (VEGF), interleukin-6, and endothelial cell-conditioned medium, they generate blast cell colonies with endothelial and primitive and definitive hematopoietic potential. The progenitor that gives rise to this colony, the blast colony-forming cell (BL-CFC) represents a transient population that is present within the EBs for approximately 24 hr, between days 2.5 and 3.5 of differentiation, preceding the onset of primitive erythropoiesis. The characteristics of the BL-CFC—namely its early development and its potential to generate primitive and/or definitive hematopoietic cells, as well as endothelial progeny—suggests that it represents the in vitro equivalent of the yolk sac hemangioblast. The protocol outlined in this chapter describes the growth of ES cells and their differentiation into two stages of development: the BL-CFC/ hemangioblast stage and the primitive/definitive hematopoietic stage.
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