Hematopoietic Aging Biomarkers in Peromyscus leucopus Mice.

Zenghua Lin,Xingmin Feng,Sachiko Kajigaya,Jichun Chen,Neal S Young

doi:10.4172/2329-8847.1000169

Abstract

We analyzed hematopoietic phenotypes in Peromyscus leucopus (PL) mice at young (2–9 months), middle (22–23 months) and old (33–46 months) ages aimed at characterizing age-associated changes in this unique rodent species. We found a significantly higher number of monocytes in old PL mice in peripheral blood, and higher proportions of CD44+ cells in blood, spleen and bone marrow in old PL mice than in middle and young counterparts. We conclude that elevated blood monocyte counts and up-regulated hematopoietic cell CD44 expression are two useful aging biomarkers for PL mice.

Highlights

Introduction and MethodsPeromyscus leucopus (PL) mice are the most populous rodent species that reside in northeastern United States [1]
The characteristic short telomere and long lifespan in PL mice provide an interesting combination that favors the utility of PL mice as a potential model for aging studies
Proportions of CD44+ cells are significantly higher in CD4 and CD8 T cells in old mice, which correspond to memory T cell accumulation [9,10,11] and gamma interferon (IFN-γ)-producing T cell enrichment [12]

Summary

Introduction

Introduction and MethodsPeromyscus leucopus (PL) mice are the most populous rodent species that reside in northeastern United States [1]. Relative to MM mice, PL mice have fewer platelets and more monocytes [6] and their peripheral blood mononuclear cells have drastically shorter telomeres [7]. Age-related phenotypic changes have been well studied in MM mouse models.

Results

Conclusion