Hematological parameters of Catfish (Clarias sp) vaccinated by Aeromonas hydrophila with different application methods

Abstract

The study aim was to find the effect of different vaccination application methods on hematological parameters of catfish (Clarias sp). A 107 Aeromonas hydrophila measured by 625 nm wave length of Spectrophotometer was applied on 25 catfish with average weight 15.83 g. The application methods were dipping (1 ml/10 l water) and intramuscular injection (0.1 ml/fish). Hematological parameter measured were hematocrit levels, total leukocytes and phagocytic index. Measurement were conduct on day 0, 21, 23 and 27 after vaccine application. The results showed that hematocrit level on fish with injection method were higher (21.02%) than dipping method (15,05%), total leukocytes on fish with dipping method were higher (3,29 x 104 cells/mm3) than injection method (3,15 x 104 cells/mm3) and phagocytic index on fish with injection were higher (25%) than dipping method (23%). The survival of catfish were also observed, and showed that catfish with injection method have higher survival rate (36%) than dipping method (34%). Aeromonas hydrophila vaccine applied with injection method have shown better immune response on catfish (Clarias sp) hematological parameters than one with dipping method.