Abstract
According to the World Health Organization, the most common (up to 90% of cases) hematological pathology is anemia, a condition characterized by a decrease in the level of hemoglobin in the blood due to a decrease in the total number of red blood cells or a violation of hemoglobin synthesis, which can have serious consequences for the body, such as decreased immunity, impaired respiratory and metabolic processes. Currently, it is necessary to improve the methods of prevention and treatment of anemia. To test new drugs for these purposes, it is necessary to create new models, including on laboratory animals. The aim of the study was to develop experimental models of iron deficiency (IDA) and hemolytic anemia (HA) on laboratory animals. The objects of the study were sexually mature male laboratory rats of the Wistar breed, aged 7 months and weighing 260 ± 20 g. 38 animals were involved in the experiment (for IDA n= 24, for HA n= 14). Laboratory animals were kept in the same external conditions for 3-4 individuals in a cage. To simulate IDA, rats were subcutaneously injected with the drug “Desferal”. To simulate GA, rats were intraperitoneally injected with the drug “Hydrochloric acid phenylhydrozine”. At each stage, blood was taken by cutting off the tip of the tail. A detailed blood test was performed on a hematological analyzer “Heska Element HT5”. As a result, it was found that when the drug “Desferal” was administered at concentrations of 125 and 250 mg / 100g per animal weight, hematological changes were observed in rats, such as an increase in red blood cells, platelets, leukocytes by stage 2 and a decrease to control values by stage 4. When the drug “Hydrochloric acid phenylhydrozine” was administered, hematological changes were detected in rats only within the experimental group. There was an increase in the indicators of erythrocytes, platelets, leukocytes by stages 2 and 3 and a decrease to control values by stage 4. Thus, when the studied drugs were administered in the presented dosages in vivo, a change in the hematological parameters of the blood was detected in the direction of an increase in their number due to adaptation to the effects of substances.
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