Hematological Investigation on One Summer Old Common Carp Feed with Fodder Supplemented with Organic Selenium

Abstract

The aim of this research is to analyze the effect of organic Selenium (Sel-Plex) on some hematological indices (hematocrit (Ht), hemoglobin (Hb), total erythrocyte count (E), total leukocyte count (Le) and determination of erythrocyte/leukocyte and lymphocyte/granulocyte ratio) and the impact of organic Selenium on the organism in one summer old common carp (Cyprinus carpio), Lausitz and Galitian varieties. The experiment were carried out on a number of 6 fish/group (randomly selected) from a group of 392 fishes, Lausitz variety, divided into 2 groups (194 fishes in the control group and 198 fishes in the experimental group) and on 6 fishes/group (randomly selected) from a total of 192 fishes, Galitian variety (94 fishes/control group and 98 fish/experimental group). The groups were reared in 2 ponds, in a polyculture semi-intensive system. The rearing conditions were identical for the 2 groups, excepting the fodder administered; the experimental group was fed with a diet containing 0.03mg/kg organic Selenium (Sel-Plex). From the correlation of the data obtained on the hematological indices and the ratios between the erythrocyte and leukocyte elements, an anemia syndrome was identified; also irritating effects were identified, influenced by the outbreak an evolution of Argulosys in the groups of one summer old common carp (both varieties). The fishes from the experimental group showed an increased immunological reaction, through a slight increase in the leukocyte numbers. Comparing the leukocytes numbers recorded in the 2 groups, a very significant difference can be observed (***P<0.001), in favor of the experimental group, Lausitz variety, compared to the control group, and significant differences (*P<0.5) in favor of the experimental group, Galitian variety, compared to the control group. The individual data were statistically analyzed using GraphPad InStat v.3 software.