Hematological, immunological and histological changes in guinea pigs in the treatment of microsporia with drugs “Micromar” and “Biogluk”

Abstract

Microsporia is a common infectious disease in the practice of a veterinarian. This disease carries a risk of human infection because it is anthropozoonotic. The main pathogen Microsporum sanis affects the upper layers of the skin and has the ability to stay on the body of an animal for up to two years. Therefore, treatment should be carried out by a complex method and be aimed at preventing the spread of the pathogen in the environment and increase the body's resistance in the fight against the disease. “Micromar” based on clotrimazole and povidone iodine and the immunostimulant “Biogluc” based on beta glucan and biotin were used to treat patients with microsporia. Clotrimazole and povidone iodine have antifungal and antiseptic properties, and beta glucan in combination with biotin stimulates the immune response and accelerates the regeneration of damaged skin. In order to determine the immune reactivity of the organism in the treatment of microsporia, hematological, immunological blood tests and histological examinations of the skin of guinea pigs infected with the pathogen M. canis were performed. The obtained results showed that when using the antifungal drug “Micromar” and immunostimulant “Biogluk” the number of leukocytes decreases from 11.13 ± 0.72 to 6.95 ± 0.10 109/L, rod-shaped neutrophils from 16.00 ± 1.29 to 6.17 ± 0.65 %, ESR from 5.67 ± 0.67 to 2.17 ± 0.31 mm/h, and the number of segmental neutrophils increases from 12.17 ± 1.47 to 22.00 ± 0, 86 %. There is a tendency to increase T-helpers. These changes indicate the development of an active immune response to the inflammatory process. In the structure of the skin there is a decrease in exudation and infiltration in the dermis, active trophism of hair follicles, which indicates the course of regenerative processes.