Hematological changes of silver carp (hypophthalmichthys molitrix) in response to Diazinon pesticide.

Aliakbar Hedayati,Elaheh Hassan Nataj Niazie

doi:10.1186/s40201-015-0208-9

Abstract

Diazinon is widely consumed for plague control in the agricultural farms and in domestic and aquaculture aspects. The present research purposed to evaluate the effect of half-lethal concentration (LC50) of diazinon on some biochemical and hematological parameters of silver carp, hypophthalmichthys molitrix, after 0, 24, 48 and 96 h. The results showed that the values of leukocytes (WBC), haematocrit (Ht), hemoglobin (Hb), MCHC, lymphocyte, cortisol and glucose were significantly increased (P < 0.05). The mount of MCV and MCH were significantly increased at 48 h and then decreased at 96 h (P < 0.05). Moreover, there was a significant increasing in neutrophils count at 48 h, and then a significant decreasing at 96 h (P < 0.05). There were no significant differences in RBC, monocyte and eosinophile counts among treatment groups at different sampling intervals. Thereupon, low-term (96 h) exposure to diazinon at half-lethal concentration (LC50) caused biochemical and hematological changes in silver carp, and offers a simply implement to appraise toxicity-derivatived changes.

Highlights

Diazinon is a vast spectrum OP, often utilized in residential areas, while its major utilization is with regard to agricultural activities
Ht and Hb levels significantly increased that was similar with results of Ahmad [11] on Cyprinus carpio in exposed to diazinon but was conversed with the findings Shaluie et al [12] on silver carp exposed to Nanocid
Our findings showed that MCV and MCH levels increased in fish exposed to 50 % LC50 diazinon at 48 h but MCHC level increased at 96 h (Fig. 2)

Summary

Introduction

Diazinon is a vast spectrum OP, often utilized in residential areas, while its major utilization is with regard to agricultural activities. Diazinon is partly water-soluble, and comes into contiguity with aquatic organisms. Afterwards, inward the cells of these organisms, diazinon is metabolized to diazoxon, which is the toxic composition of this insecticide. Diazinon affects abroad range of non-target organisms, such as invertebrates, birds, mammals and fishes, those are habiting aquatic environment [2]. Diazinon is widely utilized in the rice paddy farms of Mazandaran, Gillan, Golestan and other areas in Iran [4]. Based on reports of Bulletin of Agriculture Ministry, the annual utilization of diazinon in iran is appraised to be 3775 t [5].

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