Hematological and CD4+ T- cell count reference interval for pregnant women attending antenatal care at Hawassa University Comprehensive Specialized Hospital, Hawassa Southern Ethiopia.

Demissie Assegu Fenta,Frank T Spradley,Lealem Gedefaw Bimerew,Yidnekachew Fiseha Gebere,Wondimagegn Adissu Malko

doi:10.1371/journal.pone.0249185

Demissie Assegu Fenta, Frank T Spradley + Show 3 more

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https://doi.org/10.1371/journal.pone.0249185

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Journal: PloS one	Publication Date: Apr 8, 2021
Citations: 5	License type: CC BY 4.0

Affiliation: Hawassa University, Jimma University

Abstract

Pregnancy is a state characterized by physiological, hematological, and immunological changes. However, the reference intervals (RI) being used in clinical practice in Ethiopia are derived from non-local general populations. Therefore; this study was aimed to determine the reference interval of hematological and immunological profiles among healthy pregnant mothers attending Hawassa University Hospital. A cross-sectional study in a total of 360 healthy pregnant women was enrolled from January to April 2019, at Hawassa University hospital. Sociodemographic and obstetric data were collected using a structured questionnaire. Blood samples collected from each participant were used to define the hematological parameters. The median and 95% intervals were calculated for the immunological and hematological profiles. P-value 0.05 was considered statistically significant. A total of 360 healthy pregnant women were enrolled in this study. The age range of the participants was 18-45 years. 342(95%) were married and 270 (75%) of the participants were multigravida. The overall median CD4+ T-cell and total WBC counts (cells/mm3) were 602 and 7.58 respectively. The overall median value for lymphocytes, neutrophils, monocytes, eosinophils, and basophil count was (cells/mm3) was 2.21, 6.74, .63, .53, and 0.09 respectively. Whereas the median RBC and platelet count was 4.48×106/μLand 212×106/μL. The median value of hematological profiles in the first, second, and third trimesters was TWBC (103/μL) (7.90, 8.30, 8.65), RBC (106/μL) (4.5, 4.6, 4.62), and PLT (103/μL) (210, 209,161) respectively. The CD4 T cell count median value was (600, 598, and 591) in the first, second, and third trimesters. Significant changes were observed in hematological and immunological parameters between trimesters (P < 0.05). Significant changes were observed in hematological and immunological parameters between trimesters (P < 0.05). Considerable differences were also seen between the values in this study and other studies from Ethiopia and other countries, indicated the need for the development of local reference intervals for pregnant women.

Highlights

Regular assessment of hematological and immunological profiles is an essential and common medical practice for the evaluation of the state of health is for sick and healthy subjects [1] and used to describe fluctuations of blood analyte concentrations in well-characterized groups of individuals such as pregnant women [2, 3]
Considerable differences were seen between the values in this study and other studies from Ethiopia and other countries, indicated the need for the development of local reference intervals for pregnant women
After written informed consent was taken from study subjects, approximately 5 mL of venous blood was collected from each study participant in the morning from 8:00 am to 11:00 am using K2Ethylene Diamine Tetra acetic Acid (K2EDTA) test tube for immunological and hematological determination and plane test tube for serological testing using a sterile syringe for a screening of HIV, HBV, HCV and Syphilis and processed within 3 hours of collection

Summary

Introduction

Regular assessment of hematological and immunological profiles is an essential and common medical practice for the evaluation of the state of health is for sick and healthy subjects [1] and used to describe fluctuations of blood analyte concentrations in well-characterized groups of individuals such as pregnant women [2, 3]. The reference ranges could be important tools as biomarkers to assess disease progression or response to therapy and in the assessment of adverse reactions to therapy [4]. These parameters may vary depending on age, gender, race, environmental and genetic background [4]. The profiles can reflect the nutritional, immunological, and hemostatic status of pregnant women [5] Likewise, it is an important predictor of pregnancy outcomes [6]. ; this study was aimed to determine the reference interval of hematological and immunological profiles among healthy pregnant mothers attending Hawassa University Hospital

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