Abstract
Objectives of this study were to evaluate hematological and biochemical parameters in rats ingested toxic doses of CuSO4.5H2O. Thirty six rats divided into 3 equal groups. First group ingested orally distilled water / day for 3 months and considered as control group , second group was drenched 8 mg /kg B.W /day of CuSO4.5H2O for 3 months and considered as Treated group1 (T1). Third group was drenched 40 mg /kg B.W /day of CuSO4.5H2O for 3 months and considered as Treated group2 (T2). Blood collection was done by direct cardiac puncture every month for hematological and biochemical examinations. Hematological parameters showed that anemia caused by CuSO4.5H2O toxicity was of microcytic hypochromic type with significant increase of neutrophils and decrease of lymphocytes and blood platelets. There was significant increase in biochemical parameters , liver enzymes (GOT,GPT,ALP) and total serum protein . It concluded that CuSO4.5H2O poisoning in rats causes significant changes in blood picture and clinical enzyme
Highlights
Introduction important inCu poisoning to evaluate the liverCupper (Cu) is a mineral found in trace amount in all tissues in the body
Blood collection were done at (30, 60 and 90) days of experiment via cardiac puncture technique. 1st part of blood samples were collected in tubes containing anticoagulant (EDTA), for hematological tests while the 2nd part of blood were collected in plain tubes and used to separate serum which stored at(-20c◦), used for measurement of biochemical parameters
The values of PCV are significantly decreased in both treated group along the period of experiment compared with controls, with no significant difference between T1 and Treated group2 (T2) group at the 1st month while the 2nd and 3rd month showed significant decrease in their values respectively .In addition to that T1 group showed that no significant decrease in values between the 1st and 2nd month, with significant decrease between the 1st and 3rd month and the 2nd and 3rd month.T2 group showed significant decrease between all intervals of experiment
Summary
Objectives of this study were to evaluate hematological and biochemical parameters in rats ingested toxic doses of CuSO4.5H2O. Third group was drenched 40 mg /kg B.W /day of CuSO4.5H2O for 3 months and considered as Treated group[2] (T2). Hematological parameters showed that anemia caused by CuSO4.5H2O toxicity was of microcytic hypochromic type with significant increase of neutrophils and decrease of lymphocytes and blood platelets. There was significant increase in biochemical parameters , liver enzymes (GOT,GPT,ALP) and total serum protein. CuSO4.5H2O poisoning in rats causes significant changes in blood picture and clinical enzyme
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