Hemato-biochemical and hormonal profiles in post-partum water buffaloes (Bubalus bubalis).

Sunil Kumar,A K Balhara,Naresh Kumar,R K Sharma,Rajesh Kumar,Lukumoni Buragohain,Inderjeet Singh,S K Phulia,Daoharu Baro

doi:10.14202/vetworld.2015.512-517

Abstract

Aim:The objective of the present study was to compare serum as well as follicular fluid (FF) biochemical and hormonal profiles along with hematological parameters in postpartum estrus, anestrus, and cystic buffaloes.Materials and Methods:Postpartum buffaloes were selected in three different groups (within 40-60 days of parturition at estrus-Group-I, postpartum >90 days at anestrum-Group-II, and postpartum cystic buffaloes in Group III). The animals selected were examined for follicular wave dynamics by routine trans-rectal ultrasonography and FF was collected by transvaginal ultrasound-guided ovum pick up technique. All hematological and biochemical parameters were analyzed by automatic analyzers while hormonal profiles analyzed by commercially available ELISA kits.Results:In the present investigation, estrum and anestrum animal differ significantly in hemoglobin levels. Serum estradiol differs significantly in estrus and anestrus while no significant difference in progesterone concentration was noted among all three stages. The results of our study suggest that significant higher increase in total protein (TP), calcium and glucose values in estrum while urea, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase significantly higher in anestrum animals.Conclusion:The conclusion of the present study is that TP and albumin, calcium, urea, glucose affects oocyte development and quality.

Highlights

Among the livestock, Buffalo (Bubalus bubalis), the long-time ruminant animal contributing to the integrated farming systems, as a source of draft power, transportation, on-farm manure, meat, milk and livelihood of the farmers [1]
The conclusion of the present study is that total protein (TP) and albumin, calcium, urea, glucose affects oocyte development and quality
The result of the present study indicated that follicular fluid (FF) AST, ALT and serum lactate dehydrogenase (LDH) concentrations in anestrus were significantly higher (p

Summary

Introduction

Buffalo (Bubalus bubalis), the long-time ruminant animal contributing to the integrated farming systems, as a source of draft power, transportation, on-farm manure, meat, milk and livelihood of the farmers [1]. Interest in buffalo breeding has tremendously increased worldwide due to the fundamental role played by the species in many climatically disadvantaged agricultural systems [2]. Such adverse conditions may affect the health and reproductive efficiency of animals. FF, in part, is a transudate of serum, as surrounding cell layers permit the free diffusion of proteins of up to 500 kDa [5]. It is a complex of restricted components of serum and follicular synthesized secretions [6]. Most of literature in buffalo FF analysis is based on ovarian FF collected from morbid genitalia [7]

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Conclusion