Hemato-biochemical alterations and antioxidant status during drug-induced thyroid dysfunction in Wister rats

Abstract

This study was carried to determine the effect of induced disturbance in thyroid hormones (hyperthyroidism and hypothyroidism in animal model). In this study, sixty adult male Wister strain rats (180-200 gm) were divided into four groups represented by two control groups for both drug-induced hypothyroidism and drug-induced hyperthyroidism groups (15/each). Drug-induced hyperthyroidism group (received 0.4mg thyroxin/100g feed, with normal tap water daily), and drug-induced hypothyroidism group (received 0.2% lithium carbonate (Li2CO3) in drinking water with normal feed daily). Hematological parameters, some serum biochemical parameters, and assessment of oxidative stress represented by MDA levels in hepatic and renal tissues were assessed. Moreover, semi-quantitative scoring and histopathological findings of hepatic and renal tissues were investigated. Results revealed that any alterations in thyroid hormones levels either if hypothyroidism or hyperthyroidism, were significantly affect different clinic-pathological profile of various body function parameters, beside their indirect effect on tissue conditions that represented by histopathological tissue changes with increase in MDA levels. Referring to the obtained results, most of hematological indices, and sodium level appeared lower than normal in drug-induced hypothyroidism; beside that lipid profile and renal function were significantly raised. In contrast, most of hematological parameters, and sodium level were higher than control group findings, while kidney functions were lower than control group readings. Moreover, liver enzymes and MDA readings were significantly higher than control readings, accompanied with significant decrease in albumin levels in both drug-induced hypo- and hyperthyroidism groups assured microscopically findings of hepatic and renal histopathology.