Abstract

Hemagglutinin stem reactive antibody response in individuals immunized with a seasonal influenza trivalent vaccine.

Highlights

  • Fold increase of Ab titerTo define the antigen region recognized by the cross-reactive Abs, we used a competitive enzyme-linked immunosorbent assay (ELISA) by mixing the serial-diluted Igs from the same volume (400 microliter, μL) of sera with a well-defined HA stem mAb CR6261, which has

  • Influenza is a contagious, acute respiratory disease caused by influenza viruses

  • The Ab response to homologous Br59-HA was measured by enzyme-linked immunosorbent assay (ELISA)

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Summary

Fold increase of Ab titer

To define the antigen region recognized by the cross-reactive Abs, we used a competitive ELISA by mixing the serial-diluted Igs from the same volume (400 microliter, μL) of sera with a well-defined HA stem mAb CR6261, which has

NT Ab titer
Findings
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