Abstract
SummaryHemagglutinin of rabies virus was prepared from CVS 11 strain grown in suspension culture of BHK-21 cells maintained in a medium containing 0.4% bovine albumin and no serum. Optimal conditions for demonstration of rabies hemagglutinin included low temperature, pH 6.2, and the use of goose erythrocytes. Normal human, burro, and goat sera contained high titered nonspecific HA inhibitors which were difficult to remove. Specificity of rabies HA antigen was, however, demonstrated with antirabies hyperimmune sera which were treated with a modified kaolin adsorption technique. The same methods were used with minor modifications in the preparation of hemagglutinins of VSV-Indiana, VSV-New Jersey, Cocal, and Kern Canyon viruses.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
