Hemagglutination titer of gamma-globulin preparations for intravenous use against OEP (original endotoxin protein), protease and elastase of Pseudomonas aeruginosa (author's transl)

Abstract

Using formalinized sheep erythrocytes treated with tannic acid and coated with OEP (protein moiety of endotoxin), protease and elastase of Pseudomonas aeruginosa, passive hemagglutination titer was measured on three kinds of gamma-globulin preparations (13 lots from each maker).67%(26/39) of gamma-globuin preparations had detectable HA antibody against OEP. 97%(38/39) had HA antibody against protease, and 92%(33/36) against elastase. No significant difference was found between mean value of OEP-HA of pepsin treated and polyethyleneglycol (PEG) treated preparations. Sulfonated preparations had a tendency to have a low OEP-HA and the difference was significant (p<0.05) compared with pepsin treated preparations. In the case of protease-HA, pepsin treated treated preparation had the highest mean value and sulfonated preparation had lowest mean value. The difference was significant. No difference in elastase HA was noted among all three gamma-globulin preparations.