Abstract
AbstractBinding of an azo dye, 4′‐dimethyl amino azo benzene‐4‐carboxylic acid (DAAC) to poly(L‐lysine) (PLL) in basic aqueous solutions at 20°C has been studied. The azo dye was found to bind to PLL when its side‐chain amino groups are in the uncharged state. This was found to be a cooperative phenomenon, and the binding constant and cooperativity factor have been evaluated. The binding of the dye was found to result in a conformational transition of PLL from the α‐helix to the β‐sheet, which in turn helps in increased dye binding.
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