Abstract
The RNA helix is a fluctuating dynamic structure in solution. In this paper we report a kinetic description of the individual base-pairs in an RNA helix (the acceptor stem of Escherichia coli tRNA Phe) obtained by proton nuclear magnetic resonance spectroscopy using the saturation recovery techniques described by Johnston & Redfield (1977). We have determined the helix opening rates of each of the six Watson-Crick G·C base-pairs in the acceptor stem of partially unfolded E. coli tRNA Phe at elevated temperature in the presence of buffers, under which conditions saturation recovery is exchange-dominated and the exchange process is rate-limited by helix-coil opening.
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