Abstract
Cold atmospheric plasma jet (CAPJ) or non-thermal plasma jet has been employed in various biomedical applications based on their functions in bactericidal activity and wound healing. However, the effect of CAPJ generated by a particular composition of gases on wound closure and the underlying mechanisms that regulate wound healing signals remain elusive. In the present study, we investigated the impact of helium (He)- or a gas mixture of He and argon (He/Ar)-generated CAPJ on cell proliferation, which is a pivotal step during the wound healing process. With careful treatment duration control, He/Ar-CAPJ effectively induced keratinocyte proliferation and migration mediated through the activation of epithelial-to-mesenchymal transition (EMT) and cell cycle progression, which was evidenced by a decrease in E-cadherin levels and increases in N-cadherin, cyclin D1, Ki-67, Cdk2, and p-ERK levels. Rat wound healing studies showed that He/Ar-CAPJ treatment facilitated granulation tissue formation and mitigated inflammation in cutaneous tissue, resulting in accelerated wound closure. These findings highlight the possibility that He/Ar-CAPJ can be developed as a therapeutic agent for enhancing wound healing.
Highlights
Cold atmospheric plasma jet (CAPJ), known as non-thermal plasma jet, is mainly operated at near human body temperature (
Human keratinocyte-derived cells, i.e., HaCaT cells, were cultured with plasma-activated medium (PAM) for evaluation of cell proliferation, migration, and protein expression to extensively investigate the CAPJ interacting with cells (Figure 2A)
We investigated the molecular mechanism through which the He and argon (He/Ar)-CAPJ induces keratinocyte migration, which is a key step in cutaneous wound healing (Usui et al, 2008)
Summary
Cold atmospheric plasma jet (CAPJ), known as non-thermal plasma jet, is mainly operated at near human body temperature (
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