Abstract
BackgroundHelicobacter pylori (H. pylori) is a type I biological carcinogen, which may cause about 75% of the total incidence of gastric cancer worldwide. H. pylori infection can induce and activate the cancer-promoting signaling pathway and affect the occurrence and outcome of gastric cancer through controlling the regulatory functions of long non-coding RNAs (lncRNAs). However, we have no understanding of the prognostic worth of lncRNAs for gastric cancer patients infected with H. pylori.MethodWe screened differentially expressed lncRNAs using DESeq2 method among TCGA database. And we built the H. pylori infection-related lncRNAs regulatory patterns. Then, we constructed H. pylori infection-based lncRNAs prognostic signatures for gastric cancer patients together with H. pylori infection, via uni-variable and multi-variable COX regression analyses. Based on receiver operator characteristic curve (ROC) analysis, we evaluated the prediction effectiveness for this model.ResultsWe identified 115 H. pylori infection–related genes were differentially expressed among H. pylori–infected gastric cancer tissues versus gastric cancer tissues. Functional enrichment analysis implies that H. pylori infection might interfere with the immune-related pathways among gastric cancer tissues. Then, we built H. pylori infection–related dys-regulated lncRNA regulatory networks. We also identified 13 differentially expressed lncRNAs were associated with prognosis for gastric cancer patients together with H. pylori infection. Kaplan-Meier analysis demonstrated that the lncRNA signatures were correlated with the poor prognosis. What is more, the AUC of the lncRNA signatures was 0.712. Also, this prognostic prediction model was superior to the traditional clinical characters.ConclusionWe successfully constructed a H. pylori–related lncRNA risk signature and nomogram associated with H. pylori–infected gastric cancer patients prognosis, and the signature and nomogram can predict the prognosis of these patients.
Highlights
Gastric cancer (GC) is one of the most common cancers of gastrointestinal system
Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis has shown that these aberrantly expressed genes were significantly involved in “hsa05120: Epithelial cell signaling in H. pylori infection” and “hsa04061: Viral protein interaction with cytokine and cytokine receptor” (Figure 2B and Table S4)
Based on the KEGG enrichment items, we mapped the genes on hsa04064 KEGG graph (Figure 3C) and constructed the “Epithelial cell signaling in H. pylori infection long noncoding RNAs (lncRNAs) regulatory pattern” (Figure 3D and Table S8)
Summary
Gastric cancer (GC) is one of the most common cancers of gastrointestinal system. According to the world cancer report provided by World Health Organization (WHO), there are more than 1 million new cases of gastric cancer at 2018, together with 783,000 attributed deaths, which makes it the third leading cause of cancer-related mortality worldwide [1]. Helicobacter pylori (H. pylori) is the type I biological carcinogens, which would cause nearly 75% of the total incidence of gastric cancer worldwide [2]. Infection of H. pylori would induce or regulate the oncogenic metabolic pathways, and affect the occurrences and outcomes of gastric cancer [3]. H. pylori infection is considered to be one of the most serious risk factor and would affect the prognosis of gastric cancer patients. Helicobacter pylori (H. pylori) is a type I biological carcinogen, which may cause about 75% of the total incidence of gastric cancer worldwide. H. pylori infection can induce and activate the cancer-promoting signaling pathway and affect the occurrence and outcome of gastric cancer through controlling the regulatory functions of long noncoding RNAs (lncRNAs). We have no understanding of the prognostic worth of lncRNAs for gastric cancer patients infected with H. pylori
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