Abstract
目的：探讨Hpylori对人正常胃黏膜上皮细胞永生细胞株GES-1和人淋巴结转移胃腺癌细胞株SGC-7901的氧化性DNA损伤作用.方法：采用细菌-细胞共培养的方法,比较Hpylori作用前后GES-1和SGC-7901细胞株的形态学变化;采用激光扫描共聚焦显微镜方法,比较Hpylori作用前后GES-1和SGC-7901细胞株8-羟基脱氧鸟苷（8-OhdG）的表达.结果：Hpylori对GES-1和SGC-7901细胞株均具有损伤作用;8-OHdG表达升高,加菌组与对照组相比差别具有统计学意义（64.9396±17.8142 vs 32.3010±7.3620;102.8344±30.2632 vs 77.1336±32.3223,均P=0.000）;而且8-OHdG表达的变化程度GES-1细胞显著高于SGC-7901细胞.结论：Hpylori能够诱导GES-1和SGC-7901细胞DNA氧化性损伤显著增加;在Hpylori氧化损伤的相关研究中,更适宜选择对损伤作用敏感的GES-1细胞株作为研究对象.
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