Abstract
Background: The role of Helicobacter pylori on gastric carcinogenesis is still unclear but it is considered to predispose carriers to gastric cancer.Objective: The aim of this study is to investigate the relationship between the extent of DNA damage of normal gastric epithelial cells and H. Pylori positive & negative gastritis according to the histological diagnosis.Methods: We compared the percentage of cometed cells on the surface of gastric epithelial cells to the cells beneath gastric mucosal cells by the process of serial incubations times. DNA damage was evaluated by the alkaline single cell gel electrophoresis (comet assay) for 52 cases ,compared 19 normal individuals with 19 patients of H.Pylori positive & negative gastritis and further 14 mixed cases with different histology grading were tested by comet procedure for detection of the relationship of histological diagnosis with DNA damage (comet percentage).Results: There was a relationship between the comet percentage and the histological diagnosis. Comet percentages in specimens from case with normal histology were significantly higher than comet percentages in specimen from case with H. pylori positive gastritis.Conclusion: There was a significant increase in the percentage of cometed cells on the surface of gastric epithelial cells in both normal and infected H. pylori cells compared to the same specimen in the subsequent gastric cell layers.
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