Helicobacter pylori acyl carrier protein: Expression, purification, and its interaction with β-hydroxyacyl-ACP dehydratase

Abstract

Acyl carrier protein (ACP) is an essential component in the type II fatty acid biosynthesis (FAS II) process and is responsible for the acyl group transfer within a series of related enzymes. In this work, the ACP from Helicobacter pylori strain SS1 was cloned and the gene sequence of Hpacp was deposited in the GenBank database (Accession No.: AY904356). Two forms of HpACP (apo, holo) were successfully purified and characterized. The thermal stability of these two forms was quantitatively investigated by CD spectral analyses. The results revealed that the holo- HpACP was more stable than apo- HpACP according to the transition midpoint temperature( T m). Moreover, the interaction of HpACP with the related enzyme (β-hydroxyacyl-ACP dehydratase, HpFabZ) was determined by GST-pull down assay and surface plasmon resonance (SPR) technique in vitro, the results showed that HpACP displays a strong binding affinity to HpFabZ ( K D = 1.2 × 10 −8 M). This current work is hoped to supply useful information for better understanding the ACP features of Helicobacter pylori SS1 strain.